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Cells maintained in DPI for 1 month showed little or no mitochondrial activity, but remained viable. In conclusion, DPI treatment can be used to acutely confer a mitochondrial-deficient phenotype, which we show effectively depletes CSCs from the heterogeneous cancer cell population. These findings have significant therapeutic implications for potently targeting CSCs, while minimizing toxic side effects. We also discuss the possible implications of DPI for the aging process.

Interestingly, previous studies in C.

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